P31 - Growth Differentiation Factor (GDF-7) promotes multiple-lineage differentiation in tenogenic cultures of mesenchymal stem cells.

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Maritha Kumlin1 , Karolina Lindberg1, Li Felländer-Tsai1, Yan Li1
1 Div of Orthopaedics and Biotechnology, Dept of Clinical Science, Intervention and Technology, Karolinska Institutet

Introduction: The repair of the tendon-bone interface, which is composed of tendon, fibrocartilage, and bony attachment, remains a clinical challenge. The application of mesenchymal stem cells (MSCs), collagen-rich extracellular matrix (ECMs), as well as growth factors, has the potential to regenerate this special multiple-tissue structure through the so-called biological augmentation.
Patients and Methods: We present here an in vitro tendon regeneration model with C3H10T1/2 cells cultured on Collagen I matrix and evaluated the lineage determination effects of Growth Differentiation Factor 7 (GDF-7).
Results: We found that besides tenogenic effect, GDF-7 also stimulates the expression of osteoblastic as well as adipocytic genes. Our results indicate that GDF-7 might be a promising growth factor for the regeneration of bone-tendon interface due to its multiple-lineage stimulation effects.
Conclusions: However, the side effect on adipogenic differentiation should be of concern, as its a knows risk factor for repair failures.